I. Gomez et al., Effects of UV radiation and temperature on photosynthesis as measured by PAM fluorescence in the red alga Gelidium pulchellum (Turner) Kutzing, BOTAN MARIN, 44(1), 2001, pp. 9-16
Potosynthetic performance in response to UV radiation was measured in the r
ed alga Gelidium pulchellum Turner (Kutzing) from northern Portugal in the
laboratory. The experiments basically consisted of exposures to three diffe
rent UV radiation conditions (PAR+UV-A+UV-B; PAR+UV-A and PAR alone) using
artificial lamps and cut off foils followed by incubations at low irradianc
e of white light to determine the recovery capacity. The effects of two dif
ferent PAR backgrounds (105 and 480 mu mol photon m(-2) s(-1)) and two grow
th temperatures (15 and 25 degreesC) as additional factors were also assess
ed. The optimal quantum yield of fluorescence (F-v/F-m) decreased after 12
h exposure to PAR+UV (equivalent to a weighted UV dose closed to 150 kJ m(-
2); from photoinhibition of PSII electron transport action spectrum). The e
lectron transport was also impaired by UV radiation. The PAR background was
a key factor determining the UV responses in this species: thalli exposed
to high PAR background (480 mu mol m(-2) s(-1)) exhibited a greater degree
of photoinhibition and a slower recovery than plants irradiated by 105 mu m
ol m(-2) s(-1). On the other hand, an incubation temperature of 15 degreesC
exacerbated the effect of UV radiation on photosynthesis (increased photoi
nhibition) followed by a slowing down in the recovery kinetic compared to s
amples incubated at 25 degreesC. These results suggest that repair processe
s may be stimulated with increasing growth temperature.