Impact of tuberculosis (TB) on HIV-1 activity in dually infected patients

Active TB in HIV-1-infected subjects is associated with increased HIV-1-rel ated immunodeficiency and mortality. We assessed plasma viral load in HIV-1 -infected patients with pulmonary TB (HIV/TB) and non-TB symptomatic HIV-1- infected patients (HIV). HIV-1 load was higher in HIV/TB compared with HIV at higher CD4 counts (> 500/mul) (P < 0.01), but not at lower CD4 counts (< 500/mul). We also evaluated the status of HIV-1 gene expression in periphe ral blood mononuclear cells (PBMC) and serum from HIV/TB and CD4-matched he althy HIV-infected patients (HIV/C) by reverse transcriptase-polymerase cha in reaction over a range of CD4 (> 900/mul to < 200/mul). HIV-1 RNA in seru m and PBMC correlated to one another, and both were markedly higher in HIV/ TB compared with HIV/C with higher CD4 counts. Also, during a longitudinal study of anti-tuberculous chemoprophylaxis in HIV-1-infected patients, 10 s ubjects who developed TB had serologies before, at the time, and after the diagnosis of TB. These HIV/TB patients had an increase in viral load (avera ge 2.5-fold) at the time of diagnosis of TB (P < 0.05). Overall, these data indicate that the transcriptional activity of HIV-1 is enhanced in HIV-1-i nfected patients with active TB, especially during early HIV-1 disease. As TB often is an early HIV-1 opportunistic infection, it may particularly fav our early viral replication and dissemination, and therefore contribute to progression of HIV-1 disease.