Intracytoplasmic sperm injection increased fertilization and good-quality embryo formation in patients with non-male factor indications for in vitro fertilization: a prospective randomized study

Objective: To compare the fertilization rate and formation of good-quality embryos with conventional IVF and ICSI in patients with non-male factor inf ertility. Design: Prospective controlled study. Setting: Infertility clinic. Patient(s): Thirty-five patients with non-male factor infertility. Intervention(s): Retrieved sibling oocytes were randomly assigned to conven tional IVF or ICSI. Of sibling oocytes assigned to ICSI, only metaphase II oocytes were injected with sperm. Main Outcome Measure(s): Fertilization rate and formation of good-quality e mbryos per retrieved oocyte. Result(s): Per retrieved oocyte, ICSI resulted in better fertilization rate compared with conventional IVF (71.3% [134 of 188] vs. 57.2% [107 of 187]) . Per retrieved oocyte, ICSI also resulted in better formation of good-qual ity embryos at 48 hours after retrieval compared with conventional IVF (64. 4% [121 of 188] vs. 47.1% [88 of 187]). Conclusion(s): In IVF patients with non-male factor infertility, subjecting some sibling oocytes to ICSI increased the fertilization rate and formatio n of good-quality embryos per retrieved oocyte. It also avoided the problem of total fertilization failure in almost all cases. (C)2001 by American So ciety for Reproductive Medicine.