Changes in immunocytochemical localization of cytoskeletal proteins in boar spermatozoa after acrosome reaction induced by specific cytoskeletal inhibitors

Certain morphological changes such as rearrangement of cytoskeletal protein s of the mammalian spermatozoa are detectable during the AR. The type of ch anges differs according to the studied sperm species and follows the course of AR. Relocation of cytoskeletal structures was previously observed espec ially in the case of actin-, alpha-, gamma -tubulin- and spectrin-containin g structures. To prove these findings we used specific inhibitors of cytosk eletal proteins (eg. colcemide, cytochalasine B, nocodazole and vinblastine ). It has been shown that the AR is influenced by cytoskeletal inhibitors, but the obtained results also document that cytoskeletal proteins actin, tu bulin and spectrin play a significant role in the course of AR in vitro. Ou r results of confocal and electron microscopy also demonstrate visible chan ges of actin-, tubulin- and spectrin-containing structures after the AR. Ou r data indicate that specific cytoskeletal inhibitors influence the AR and they prove the role of cytoskeletal proteins in this process.