An integrated physical map of 8q22-q24: Use in positional cloning and deletion analysis of Langer-Giedion syndrome

We have developed an integrated map for a 35-cM area of human chromosome 8 surrounding the Langer-Giedion syndrome deletion region, This map spans fro m approximately 8q22 to 8q24 and includes 10 hybrid cell intervals, 89 poly morphic STSs, 118 ESTs, and 37 known genes or inferred gene homologies. The map locations of 25 genes including osteoprotegerin, syndecan-2, and autot axin have been refined from the general locations previously reported. In a ddition, the map has been used to indicate the location of nine deletions i n patients with Langer-Giedion syndrome and trichorhinophalangeal syndrome type I to demonstrate the potential usefulness of the map in the analysis o f these complex syndromes. The map will also be of interest to anyone tryin g to clone positionally disease genes in this region, such as Cohen syndrom e (8q22-q23), Klip-Feil syndrome (8q22.2), hereditary spastic paraplegia (8 q24), and benign adult familial myoclonic epilepsy (8q23.3-q24.1). (C) 2001 Academic Press.