T. Sawada et al., Cross-linked hyaluronate hydrogel prevents adhesion formation and reformation in mouse uterine horn model, HUM REPR, 16(2), 2001, pp. 353-356
The aim of this study was to evaluate the efficacy of cross-linked hyaluron
ate hydrogel (HA gel) as an adjuvant for postoperative adhesion prevention,
in a mouse uterine horn model. In experiment 1 uterine horns were abrased
with iodine. HA gel was applied to the injured surface before closure in th
e treatment group. In experiment 2, after injuring the uterine horns, three
stitches were placed at equal distances around the uterine horns to appose
the injured medial surfaces of the two horns during healing. HA gel was in
serted between the uterine horns in the treatment group. In experiment 3 pr
evention of adhesion reformation was assessed. After lysis of adhesions tha
t were induced as in experiment 2, HA gel was introduced between the serosa
l surfaces of apposing uterine horns. Untreated animals served as controls
in each experiment. Statistical analysis was carried out using Student's t-
test. The adhesion score was significantly lower in the HA gel group on the
14th day compared with controls in all the experiments: in experiment 1, 0
.3 +/- 0.4 versus 1.7 +/- 1.2; in experiment 2, 0.9 +/- 1.0 versus 2.6 +/-
0.5; and in experiment 3, 1.5 +/- 0.9 versus 2.2 +/- 0.6 respectively. Cros
s-linked HA gel significantly reduced de-novo adhesions (P < 0.03) and adhe
sion reformation (P < 0.03).