Introduction: Secondary cataract formation limits visual function after cat
aract surgery. Various experimental methods utilizing the pharmacologic inh
ibition of lens epithelial cell proliferation have been proposed. However,
diffusion into the anterior chamber may lead to damage of corneal endotheli
al cells. This study evaluated the inhibition of lens epithelial cell proli
feration with a capsular bag ring, labeled with a beta -emitting radioisoto
pe.
Methods and Materials: In vitro studies using rabbit lens epithelial cells
were performed to investigate the dose-dependent effect of irradiation. Bas
ed on these results, P-32-labeled PMMA rings were implanted into the capsul
ar bag of NZW rabbits in vivo after phacoemulsification. Animals were exami
ned for development of posterior capsule opacification over a period of 12
weeks following surgery. Radiation damage to the surrounding ocular tissue
was subsequently analyzed in histologic sections using TUNEL assay and prol
iferation marker.
Results: Irradiation of lens epithelial cells in vitro with >5 Gy resulted
in a dose-dependent decrease in the number of cells. BrdU testing demonstra
ted a near complete inhibition of cell proliferation, In vivo, implantation
of P-32-1abeled PMMA rings led to inhibition of epithelial cell proliferat
ion and secondary cataract formation but was not able to fully inhibit aber
rant differentiation of some remaining cells. Histologic examination showed
no evidence of radiation damage of the ciliary body or the corneal endothe
lium,
Conclusions: Low-dose beta irradiation exhibits the potential for inhibitio
n of lens epithelial cell proliferation both in vitro and in vivo, Further
investigation of various nuclides and their radiation profiles is needed to
optimize the prevention of posterior capsule opacification due to epitheli
al cell proliferation. (C) 2001 Elsevier Science Inc.