Crossbred steers (n=136) were used to assess breed differences in growth pe
rformance, carcass characteristics, fatty acid composition (total lipids an
d phospholipids), and palatability attributes of longissimus muscle. A mult
iple regression model was applied to crossbreeding data to estimate genetic
differences between Simmental and Red Angus at the same level of backfat f
inish (10 mm). Simmental spent 71 more (P < 0.001) days on feed to acquire
the same degree of backfat thickness as Red Angus, had heavier (P < 0.001)
slaughter weights, larger (P = 0.002) longissimus muscle area, and increase
d (P = 0.023) lean yield. Average daily gain did not differ (P = 0.297) bet
ween breeds. Simmental were less (P = 0.012) efficient in converting feed t
o gain than Red Angus. Generally, there were few breed differences in palat
ability attributes for longissimus and semitendinosus muscles, with the exc
eption of increased (P < 0.05) beef flavor scores for Simmental beef vs Red
Angus beef across both muscles. For total lipids, concentrations of myrist
oleic acid (14:1), palmitoleic acid (16:1), and vaccenic acid (18:1n-7), al
ong with n-6 to n-3 fatty acid (n-6:n-3) ratio, were greater (P < 0.05) in
Simmental than Red Angus. In contrast, concentrations of margaric acid (17:
0), eicosapentaenoic acid (20:5n-3), and total n-3 polyunsaturated fatty ac
ids (n-3 PUFA) were greater (P < 0.05) in Red Angus than Simmental. For pho
spholipids, Simmental had lower (P < 0.05) amounts of 20:5n-3, docosahexaen
oic acid (22:6n-3), and n-3 PUFA, with a greater (P = 0.017) n-6:n-3 ratio.
Activity of Delta (9)-desaturase enzyme in the conversion of palmitic acid
(16:0) to 16:1 was greater (P = 0.001) in total lipids from Simmental as c
ompared with Red Angus. A genetic basis for fatty acid differences is sugge
sted, although the biological and practical significance needs to be demons
trated.