Improved determination of urinary oxalate with alkylamine glass bound barley oxalate oxidase

Authors
Chandran, P Thakur, M Pundir, CS
Citation
P. Chandran et al., Improved determination of urinary oxalate with alkylamine glass bound barley oxalate oxidase, J BIOTECH, 85(1), 2001, pp. 1-5
Citations number
10
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOTECHNOLOGY
ISSN journal
01681656 → ACNP
Volume
85
Issue
1
Year of publication
2001
Pages
1 - 5
Database
ISI
SICI code
0168-1656(20010123)85:1<1:IDOUOW>2.0.ZU;2-V
Abstract
The measurement of oxalate in urine was improved by employing barley oxalat e oxidase immobilized on alkylamine glass beads affixed in a glass beaker. The minimum detection limit was 3.6 mg l(-1) urine. The recovery of added o xalate was 88.9 +/- 9.2%. Within- and between-assay coefficients of variati on (CV) were < 4.0 and < 9.4%, respectively. The urinary oxalate values wer e obtained by a commercial kit method and the present method showed a good correlation (0.999). The method is free from tedious handling of glass bead s and Cl- interference. (C) 2001 Elsevier Science B.V. All rights reserved.