M. Touraki et al., High-performance liquid chromatographic determination of oxolinic acid andflumequine in the live fish feed Artemia, J CHROMAT B, 751(2), 2001, pp. 247-256
A high-performance liquid chromatography (HPLC) analytical method for the d
etermination of oxolinic acid and flumequine in Artemia nauplii is describe
d. The samples were extracted and cleaned up by a solid-phase extraction (S
PE) procedure using SPE C-18 cartridges. Oxolinic acid and flumequine were
determined by reversed-phase HPLC using a mobile phase of methanol-0.1 M ph
osphate buffer, pH 3 (45:55, v/v) and a UV detection wavelength of 254 nm.
Calibration curves were linear for oxolinic acid in the range of 0.2-50 mug
/g (r(2)=0.9998) and for flumequine in the range of 0.3-50 mug/g (r(2)=0.99
94). Mean recoveries amounted to 100.8% and 98.4% for oxolinic acid and flu
mequine, respectively. The quantification limit was 0.2 mug/g for oxolinic
acid and 0.3 mug/g for flumequine. Quantitative data from an in vivo feedin
g study indicated excellent uptake of both drugs by Artemia nauplii. (C) 20
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