This study used a previously developed model to predict herd mean energy ba
lance of the first 12 wk of lactation from test-day information. The predic
tions were compared with calculated energy balance based on feed analysis a
nd to changes in body weight. Seven independent feeding trials including 43
diets (519 lactations, 254 cows; 1987 to 1996) were used. Conventional die
ts were discriminated from nonconventional diets by significant differences
between mean calculated energy balance of subtrial diets versus control di
ets. The total difference between group means of predicted minus calculated
energy balance was positive throughout the observed lactation period. It w
as lowest (5 to 9 MJ of net energy for lactation) during negative energy ba
lance of the conventional diets in wk 2 to 7 when 18 to 50% of the total di
fference was due to random variation. Because of this difference, both pred
icted and calculated energy balances were compared to body weight change as
a reference for true energy balance. Body weight change was adjusted for r
umen fill. While calculated energy balance tended to be negative at times w
hen cows gained weight, predicted energy balance was positive. Cows fed non
conventional diets gained weight, while calculated energy balance was extre
mely negative, whereas predicted energy balance based on test-day informati
on was positive. We concluded that the prediction difference was relatively
small when standard rations were used, and that nonconventional rations bi
ased predicted energy balance to a lesser extent than calculated energy bal
ance. Estimating energy balance based on test-day information appears feasi
ble.