Regulation of inhibitory and activating killer-cell Ig-like receptor expression occurs in T cells after termination of TCR rearrangements

Citation
F. Vely et al., Regulation of inhibitory and activating killer-cell Ig-like receptor expression occurs in T cells after termination of TCR rearrangements, J IMMUNOL, 166(4), 2001, pp. 2487-2494
Citations number
54
Categorie Soggetti
Immunology
Journal title
JOURNAL OF IMMUNOLOGY
ISSN journal
00221767 → ACNP
Volume
166
Issue
4
Year of publication
2001
Pages
2487 - 2494
Database
ISI
SICI code
0022-1767(20010215)166:4<2487:ROIAAK>2.0.ZU;2-R
Abstract
A small fraction of T cells expresses killer-cell Ig-like receptors (KIR), a family of MHC class I-specific receptors that can modulate TCR-dependent activation of effector functions. Although KIR+ cells are enriched within A g-experienced T cell subsets, the precise relationships between KIR+ and KI R- T cells and the stage of KIR induction on these lymphocytes remain uncle ar. In this study, we compared KIR- and KIR+ alpha beta T cell clones, sort ed by means of the CD158b (KIR2DL2/KIR2DL/KIR2DS2) specific mAb GL183. We i solated several pairs of CD158b(+) and CD158b(-) alpha beta T cell clones s haring identical productive and nonproductive TCR transcripts. We showed th at expression of functional KIR on T cells is regulated after termination o f TCR rearrangements. Transcriptional regulation of KIR genes was documente d in multiple T cell clones generated from the same donor, and the presence of KIR transcripts was also detected in KIR- T cells. These results docume nt a complex regulation of KIR expression in T cells at both pre and posttr anscriptional levels, under the control of yet undefined signals provided i n vivo. The Journal of Immunology, 2001, 166: 2487-2494.