F. Vely et al., Regulation of inhibitory and activating killer-cell Ig-like receptor expression occurs in T cells after termination of TCR rearrangements, J IMMUNOL, 166(4), 2001, pp. 2487-2494
A small fraction of T cells expresses killer-cell Ig-like receptors (KIR),
a family of MHC class I-specific receptors that can modulate TCR-dependent
activation of effector functions. Although KIR+ cells are enriched within A
g-experienced T cell subsets, the precise relationships between KIR+ and KI
R- T cells and the stage of KIR induction on these lymphocytes remain uncle
ar. In this study, we compared KIR- and KIR+ alpha beta T cell clones, sort
ed by means of the CD158b (KIR2DL2/KIR2DL/KIR2DS2) specific mAb GL183. We i
solated several pairs of CD158b(+) and CD158b(-) alpha beta T cell clones s
haring identical productive and nonproductive TCR transcripts. We showed th
at expression of functional KIR on T cells is regulated after termination o
f TCR rearrangements. Transcriptional regulation of KIR genes was documente
d in multiple T cell clones generated from the same donor, and the presence
of KIR transcripts was also detected in KIR- T cells. These results docume
nt a complex regulation of KIR expression in T cells at both pre and posttr
anscriptional levels, under the control of yet undefined signals provided i
n vivo. The Journal of Immunology, 2001, 166: 2487-2494.