A new matrix for membrane affinity chromatography and its application to the purification of concanavalin A

Authors
Guo, W Ruckenstein, E
Citation
W. Guo et E. Ruckenstein, A new matrix for membrane affinity chromatography and its application to the purification of concanavalin A, J MEMBR SCI, 182(1-2), 2001, pp. 227-234
Citations number
21
Categorie Soggetti
Chemistry,"Chemical Engineering
Journal title
JOURNAL OF MEMBRANE SCIENCE
ISSN journal
03767388 → ACNP
Volume
182
Issue
1-2
Year of publication
2001
Pages
227 - 234
Database
ISI
SICI code
0376-7388(20010215)182:1-2<227:ANMFMA>2.0.ZU;2-S
Abstract
Macroporous cellulose membranes with large pore sizes (1-2 mum) and high po rosity (about 60%) were prepared from filter paper by chemical crosslinking . These membranes were activated by introducing epoxy groups with various s pacer lengths. after which maltose was immobilized as an affinity ligand. p acked into a cartridge, the prepared membranes were used for the affinity p urification of concanavalin A. (C) 2001 Elsevier Science B.V. All rights re served.