Validation of a CE assay for the analysis of isomeric aminopyridines and diaminopyridines

A capillary electrophoresis assay for the analysis of aminopyridines and di aminopyridines has been developed and validated. The compounds were separat ed using a 100 mM sodium acetate buffer at pH 5.15, an applied voltage of 2 0 kV and a capillary of 60 cm effective length. N-(1-naphthyl)ethylenediami ne was used as internal standard to compensate for injection errors and min or fluctuations of the migration times. The detection wavelength was set at 240 nm and not optimized for a specific derivative. The assay was validate d with respect to specificity, linearity, range, limit of quantitation and detection, precision, and robustness. Within certain limits, the assay also allowed the detection and determination of the other aminopyridine derivat ives at the 0.1% level as demonstrated for 3,4-diaminopyridine. (C) 2001 El sevier Science B.V. All rights reserved.