Pore properties and ionic block of the rabbit epithelial calcium channel expressed in HEK 293 cells

1. We have used the whole-cell patch-clamp technique to analyse the permeat ion properties and ionic block of the epithelial Ca2+ channel ECaC heterolo gously expressed in human embryonic kidney (HEK) 293 cells. 2. Cells dialysed with 10 mM BAPTA and exposed to Ca2+-containing, monovale nt cation-free solutions displayed large inwardly rectifying currents. Thei r reversal potential depended on the extracellular Ca2+ concentration, [Ca2 +](o). The slope of the relationship between reversal potential and [Ca2+]( o) on a logarithmic scale was 21 +/- 4 mV, compared with 29 mV as predicted by the Nernst equation (n = 3-5 cells). 3. Currents in mixtures of Ca2+ and Na+ or Ca2+ and Ba2+ showed anomalous m ole fraction behaviour. We have described the current-concentration plot fo r Ca2+ and Na+ by a kinetic permeation model, i.e, the 'step' model. 4. Extracellular Mg2+ blocked both divalent and monovalent currents with an IC50 of 62 +/- 9 muM (n = 4) in Ca2+-free conditions and 328 +/- 50 muM (n = 4-9) in 100 muM Ca2+ solutions. 5. Mono- and divalent currents through ECaCs were blocked by gadolinium, la nthanum and cadmium, with a blocking order of Cd2+ >> Gd3+ > La3+. 6. We conclude that the permeation of monovalent and divalent cations throu gh ECaCs shows similarities with L-type voltage-gated Ca2+ channels, the ma in differences being a higher Ca2+ affinity and a significantly higher curr ent density in micromolar Ca2+ concentrations in the case of ECaCs.