Jm. Trifaro et al., SECRETORY VESICLE POOLS AND RATE AND KINETICS OF SINGLE VESICLE EXOCYTOSIS IN NEUROSECRETORY-CELLS, Neurochemical research, 22(7), 1997, pp. 831-841
Secretory vesicles are localized in specific compartments within neuro
secretory cells. Morphometric, cytochemical and electrophysiological t
echniques have allowed the definition of secretory Vesicle compartment
s. These are different pools in which vesicles are in various states o
f releasability. The transit of vesicles between compartments is not r
andom, but an event controlled and regulated by Ca2+ and the cortical
F-actin network. Cortical F-actin disassembly, a Ca2+-dependent event,
controls the transit of secretory vesicles from the reserve compartme
nt to the release-ready vesicle pool. Furthermore, the recent developm
ent of new technical approaches (patch-clamp membrane capacitance, ele
ctrochemical detection of amines with carbon-fibre microelectrodes) ha
s now permitted us to understand the kinetics of single vesicle exocyt
osis.