Streptogramin resistance and shared pulsed-field gel electrophoresis patterns in vanA-containing Enterococcus faecium and Enterococcus hirae isolatedfrom humans and animals in Spain
B. Robredo et al., Streptogramin resistance and shared pulsed-field gel electrophoresis patterns in vanA-containing Enterococcus faecium and Enterococcus hirae isolatedfrom humans and animals in Spain, MICROB DR R, 6(4), 2000, pp. 305-311
The present study was performed to determine if;any of the 45 vanA-containi
ng Enterococcus faecium or 18 vanA-containing E. hirae strains were shared
by chickens (32 E. faecium/17 E. hirae) and humans (13 E. faecium/1 E, hira
e) using pulsed field gel electrophoresis (PFGE) and to study quinupristin-
dalfopristin (Q-D) resistance. Seven of the 45 E, faecium isolates (from 2
outpatients and from 5 poultry products) were resistant to Q-D (MIC greater
than or equal to 16 mug/ml); one strain was shown to have satA by PCR and
sequencing and, in the other six isolates, the recently described satG gene
was demonstrated. Six different PFGE patterns were detected among the 7 Q-
D E. faecium-resistant isolates. None of the E. hirae isolates showed Q-D r
esistance. Among the 45 vanA-containing E. faecium strains, 25 unrelated cl
ones were found by PFGE with highly diverse patterns and an indistinguishab
le PFGE pattern was observed in vanA-containing E. faecium strains from two
humans and two poultry products. A single PFGE pattern was detected in 17
of 18 vanA-containing E. hirae isolates, obtained from one human and 16 chi
cken samples. Based on the presence of indistinguishable PFGE patterns amon
g VR E. faecium and E. hirae from humans and chickens, we conclude that hor
izontal transfer of these strains could occur between both groups.