Establishment of a PCR-RFLP library for basidiomycetes, ascomycetes and their ectomycorrhizae on Picea abies (L.) Karst.

Citation
T. Grebenc et al., Establishment of a PCR-RFLP library for basidiomycetes, ascomycetes and their ectomycorrhizae on Picea abies (L.) Karst., PHYTON AUST, 40(4), 2000, pp. 79-82
Citations number
8
Categorie Soggetti
Plant Sciences
Journal title
PHYTON-ANNALES REI BOTANICAE
ISSN journal
00792047 → ACNP
Volume
40
Issue
4
Year of publication
2000
Pages
79 - 82
Database
ISI
SICI code
0079-2047(2000)40:4<79:EOAPLF>2.0.ZU;2-D
Abstract
The internal transcribed spacer (ITS) regions in ribosomal genes are variab le enough to distinguish most mycorrhizal and other higher fungi to the spe cies level. Polymerase chain reaction in combination with restriction fragm ent length polymorphism (PCR-RFLP) can be used to establish the digestion p attern library based on fungal sporocarps. We established the digestion pat tern library for over 150 spp of mycorrhizal and nonmycorrhizal higher fung i predominantly collected in a four year period on a 1 ha permanent forest research plot of the Slovenian Forestry Institute in an autochthonous altim ontane Norway spruce forest on Pokljuka (Triglav national park, 1200m above see level). Only the problematic of genus Russula is presented here. The s ame method is being used on mycorrhizal root tips from the same plot for de termination of types of ectomycorrhizae.