Microbiological testing for Campylobacter and Salmonella was performed at a
processing plant for squab (young pigeon) in three separate trials. Live b
irds, carcasses, and equipment were targeted for sampling during the presla
ughter, pre-evisceration, and postevisceration stages of processing. The th
ree trials represented 18 farms (1,110 squab), 1 farm (250 squab), and 23 f
arms (2,900 squab). The overall prevalence of positive samples in Trial 1 w
as 1.4% for Salmonella spp. and 11.1% for C. jejuni; in Trial 2, 4.3 and 0%
for Salmonella spp. and C. jejuni; and in Trial 3, 4.1 and 4.8% for Salmon
ella spp. and C. jejuni, respectively. These observations represent a signi
ficantly greater likelihood of having a positive sample for Campylobacter (
twofold) or Salmonella (eightfold) at processing, compared with prevalences
observed in our previous on-farm study. This finding suggests an overall i
ncrease in the number of carcasses contaminated or in the concentration of
contamination during transport and processing. In the multifarm trials, onl
y Trial 3 demonstrated a significant increase in the prevalence of positive
samples from the preslaughter to the postevisceration stages of processing
(P = 0.02), and only for Campylobacter. The prevalence of positive culture
s from equipment surfaces were not different than carcasses during processi
ng, therefore no additional critical control points were identified within
this system. When pooled swabs were compared (Trial 1) to individual swabs
(Trials 2 and 3), no statistical difference in the prevalence of Salmonella
or Campylobacter was observed between trials. Direct plating from a pooled
sample onto selective agar media (Trial 1) and single swab culture with en
richment followed by plating on selective agar (Trials 2 and 3) were compar
ed for Campylobacter isolation. No statistical difference in C. jejuni prev
alence was observed using either method; however, when the detection limit
of each method was determined, single swabs with enrichment had greater sen
sitivity.