M. Santillan et Mc. Mackey, Dynamic regulation of the tryptophan operon: A modeling study and comparison with experimental data, P NAS US, 98(4), 2001, pp. 1364-1369
Citations number
19
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
A mathematical model for regulation of the tryptophan operon is presented.
This model takes into account repression, feedback enzyme inhibition, and t
ranscriptional attenuation, Special attention is given to model parameter e
stimation based on experimental data. The model's system of delay different
ial equations is numerically solved, and the results are compared with expe
rimental data on the temporal evolution of enzyme activity in cultures of E
scherichia coli after a nutritional shift (minimal + tryptophan medium to m
inimal medium). Good agreement is obtained between the numeric simulations
and the experimental results for wild-type E. coli. as well as for two diff
erent mutant strains.