Individual expression of recombinant alpha- and beta-tubulin from Haemonchus contortus: Polymerization and drug effects

Three tubulin isotypes from the parasitic nematode Haemonchus contortus wer e individually expressed in Escherichia coli, purified, and induced to poly merize into microtubules in the absence of microtubule-associated proteins. The effect of different conditions on the rate of polymerization of pure t ubulin was assessed. This is the first time that recombinant alpha -tubulin has been shown to be capable of polymerization into microtubule-like struc tures when incubated with recombinant P-tubulin. In addition, the present s tudy has shown that: (1) microtubule-associated proteins are not required f or tubulin polymerization; and (2) pure beta -tubulin isotype, beta 12-16, alone was capable of forming microtubule-like structures in the absence of alpha -tubulin. Polymerization of the recombinant invertebrate tubulin, as measured by a spectrophotometric assay, was found to be enhanced by a conce ntration of tubulin >0.25 mg/mL; temperature greater than or equal to 20 de greesC; 2 mM GTP; glycerol; EGTA; and Mg2+. Polymerization was inhibited by GTP (>2 mM) and albendazole. Calcium ions and a pH range of 6 to 8.5 had n o measurable effect on polmerization. Individual isotypes of tubulin polyme rized to approximately the same extent as an alpha-/beta- tubulin mixture. Samples of tubulin assembled under the above conditions for 60 min were als o examined under a transmission electron microscope. Although the spectroph otometric assay indicated polymerization, it did not predict the structure of the polymer. In many cases tubulin sheets, folded sheets, and rings were observed in addition to, or instead of, microtubule-like structures. (C) 2 001 Academic Press.