ITA
ENG

A high-resolution radiation hybrid map of the human genome draft sequence

Authors

Olivier, M Aggarwal, A Allen, J Almendras, AA Bajorek, ES Beasley, EM Brady, SD Bushard, JM Bustos, VI Chu, A Chung, TR De Witte, A Denys, ME Dominguez, R Fang, NY Foster, BD Freudenberg, RW Hadley, D Hamilton, LR Jeffrey, TJ Kelly, L Lazzeroni, L Levy, MR Lewis, SC Liu, X Lopez, FJ Louie, B Marquis, JP Martinez, RA Matsuura, MK Misherghi, NS Norton, JA Olshen, A Perkins, SM Perou, AJ Piercy, C Piercy, M Qin, F Reif, T Sheppard, K Shokoohi, V Smick, GA Sun, WL Stewart, EA Tejeda, JF Tran, NM Trejo, T Vo, NT Yan, SCM Zierten, DL Zhao, SY Sachidanandam, R Trask, BJ Myers, RM Cox, DR

Citation

M. Olivier et al., A high-resolution radiation hybrid map of the human genome draft sequence, SCIENCE, 291(5507), 2001, pp. 1298

Citations number

Categorie Soggetti

Multidisciplinary,Multidisciplinary,Multidisciplinary

Journal title

SCIENCE

ISSN journal

00368075 → ACNP

Volume

291

Issue

5507

Year of publication

2001

Database

ISI

SICI code

0036-8075(20010216)291:5507<1298:AHRHMO>2.0.ZU;2-S

Abstract

We have constructed a physical map of the human genome by using a panel of 90 whole-genome radiation hybrids (the TNG panel) in conjunction with 40,32 2 sequence-tagged sites (STSs) derived from random genomic sequences as wel l as expressed sequences. Of 36,678 STSs on the TNC radiation hybrid map, o nly 3604 (9.8%) were absent from the unassembled draft sequence of the huma n genome. Of 20,030 STSs ordered on the TNG map as well as the assembled hu man genome draft sequence and the Celera assembled human genome sequence, 3 6% of the STSs had a discrepant order between the working draft sequence an d the Celera sequence. The TNG map order was identical to one of the two se quence orders in 60% of these discrepant cases.