GENETIC-MAPPING OF A POSSIBLE NEW ALCOHOL-DEHYDROGENASE SEQUENCE TO MOUSE CHROMOSOME-3 AT THE ADH-1 ADH-3 COMPLEX/

Southern blot analysis of mouse genomic DNA reveals two EcoRI fragment s which faintly hybridize to mouse Adh-l cDNA and are not part of the Adh-1 gene, These fragments were isolated from agarose gels, cloned an d characterized. Sequence analysis of the 2.1-kb EcoRI fragment sugges ts that it is likely a pseudogene since it does not contain a long ope n reading frame. However; the 2.0-kb EcoRI fragment contains a coding sequence with a long open reading frame which corresponds to exon 6 of the mouse Adh-l gene. Comparison of the coding sequence with other kn own ADHs suggests that the sequence has diverged sufficiently from any currently known class of ADH to be a possible distinct class. Further confirmation, awaits analysis of currently available genomic clones. Using these sequences as probe, restriction fragment length polymorphi sms were identified for each sequence between C57B1/6J and DBA/2J inbr ed mouse strains, The strain distribution pattern for these allelic di fferences was determined among the B x D recombinant inbred strains. T his analysis revealed that the 2.1-kb EcoRI sequence is located on chr omosome 3 but at a distance from the Adh-1/Adh-3 complex as previously reported. However the new polymorphism identified in the 2.0-kb EcoRI fragment enabled this sequence to be mapped at the Adh-1/Adh-3 comple x.