Identification of a novel DRB1 allele, DRB1*1112, by sequence-based DRB typing

We report here a novel DRB1 allele (DRB1*1112) identified during sequence-b ased HLA-DRB typing. Polymerase chain reaction with generic DRB primers and group-specific primers and subsequent sequencing yielded identical results . Molecular cloning and sequencing confirmed that the new DRB1 allele is id entical to DRB1*11011 and 1129 at exon 2 except for a single nucleotide sub stitution at codon 37, changing the codon from Tyr (DRB1*11011) or Ser (DRB 1*1129) to Phe.