Genetic polymorphisms as biomarkers of sensitivity to inhaled sulfur dioxide in subjects with asthma

Background: Individuals with asthma are sensitive to inhaled sulfur dioxide (SO2); decrements in pulmonary function occur after exposure to low concen trations even for a short duration of time. There is a great amount of inte rindividual variation in response to SO2. Objective: It was our objective to determine whether one of the following p olymorphism locations linked with asthma is associated with the bronchial h yperresponsiveness to SO2 observed in some asthmatic patients: the beta (2) -adrenergic receptor, interleukin-4 (IL-4) receptor alpha subunit, Clara ce ll secretory protein (CC16), TNF-alpha gene promoter, and first intron of t he lymphotoxin alpha (LT-alpha) gene. Methods: Subjects were volunteers with physician-diagnosed asthma requiring regular asthma medication. Spirometry was performed before and after a 10- minute exposure to 0.5 ppm SO2. Subjects were classified as SO2 responders if forced expiratory volume in 1 second (FEV1) decreased greater than or eq ual to 12%. DNA obtained from buccal cell samples was analyzed for genetic polymorphisms. Results: Of the 62 subjects (21 male and 41 female), 13 had a 12% or greate r decrement in FEV1 after SO2 exposure (range +19% to -49%). Response to SO 2 was associated with the wild-type allele of the TNF-alpha promoter polymo rphism (12 of 12 SO2 responders versus 28 of 46 nonresponders; P < .05) but with no other polymorphisms. Medication category and atopic status showed no association with SO2 sensitivity. Conclusions: The wild-type allele of the TNF-<alpha> promoter polymorphism may be associated with mechanisms of asthmatic sensitivity to inhaled SO2.