Characterization and transcriptional analysis of Pseudomonas fluorescens denitrifying clusters containing the nar, nir, nor and nos genes

Authors
Philippot, L Mirleau, P Mazurier, S Siblot, S Hartmann, A Lemanceau, P Germon, JC
Citation
L. Philippot et al., Characterization and transcriptional analysis of Pseudomonas fluorescens denitrifying clusters containing the nar, nir, nor and nos genes, BBA-GENE ST, 1517(3), 2001, pp. 436-440
Citations number
21
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1517
Issue
3
Year of publication
2001
Pages
436 - 440
Database
ISI
SICI code
0167-4781(20010216)1517:3<436:CATAOP>2.0.ZU;2-H
Abstract
In this study, we report the cloning and characterization of denitrifying g ene clusters of Pseudomonas fluorescens C7R12 containing the narXLDKGHJI, n ir POQSM. norCB and nosRZDFYL genes. While consensus sequences for Fnr-like protein binding sites were identified in the promoter regions of the nar, nir, nor and nos genes, consensus sequences corresponding to the NarL bindi ng sites were identified only upstream the nar genes. Monitoring by mRNA an alysis the expression of the narG.,nirS, norB and nosZ structural genes sug gests a sequential induction of the denitrification system in P. fluroescen s. (C) 2001 Elsevier Science B.V. All rights reserved.