Identification of a murine cysteinyl leukotriene receptor by expression inXenopus laevis oocytes

Authors
Mollerup, J Jorgensen, ST Hougaard, C Hoffmann, EK
Citation
J. Mollerup et al., Identification of a murine cysteinyl leukotriene receptor by expression inXenopus laevis oocytes, BBA-GENE ST, 1517(3), 2001, pp. 455-459
Citations number
19
Categorie Soggetti
Molecular Biology & Genetics
Journal title
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION
ISSN journal
01674781 → ACNP
Volume
1517
Issue
3
Year of publication
2001
Pages
455 - 459
Database
ISI
SICI code
0167-4781(20010216)1517:3<455:IOAMCL>2.0.ZU;2-8
Abstract
We report the identification of an EST encoding a murine cysteinyl leukotri ene (mCysLT) receptor. LTD4, LTC4 and LTE4 but not LTB4 or various nucleoti des activated Ca2+-evoked Cl- currents in mCysLT(1) expressing Xenopus laev is oocytes. The response to LTD4 was blocked by MK-571, reduced by pretreat ment with pertussis toxin (PTX), and was partly dependent on extracellular Ca2+. The identified murine CysLT(1) receptor differs from the hCysLT(1) re ceptor with regard to PTX sensitivity, receptor-mediated Ca2+ influx, and a ntagonist sensitivity. (C) 2001 Elsevier Science B.V. All rights reserved.