Nucleotide and deduced amino acid sequences of an alkaline pullulanase from the alkaliphilic bacterium Bacillus sp KSM-1876

The nucleotide sequence of an alkaline pullulanase-encoding gene from alkal iphilic Bacillus sp. strain KSM-1876 was determined. The open reading frame of the gene encoded 1142 amino acids with a calculated molecular mass of 1 28 739 Da. The alkaline pullulanase showed very limited homology ( < 32% id entity) to previously reported debranching enzymes from prokaryotes and euk aryotes. It contained unique tandem repeats in both the N-terminal and the C-terminal regions. (C) 2001 Elsevier Science B.V. All rights reserved.