G. Catapano et al., Morphology and metabolism of hepatocytes cultured in Petri dishes on filmsand in non-woven fabrics of hyaluronic acid esters, BIOMATERIAL, 22(7), 2001, pp. 659-665
Polymers of hyaluronic acid (Hyal) esters exhibit good tissue compatibility
and are available in various geometrical configurations. These properties
can be exploited for the design of innovative bioartificial liver support d
evices (BALSDs) using primary hepatocytes. In this paper, we report a preli
minary investigation of the polymer feasibility of the ethyl and the benzyl
Hyal ester in the form of films and non-woven Fabrics for the in vitro cul
ture of primary rat hepatocytes. Cell function was evaluated daily in Petri
dishes with respect to the rate of ammonia elimination (AER) and urea synt
hesis (USR). Cells cultured in non-woven fabrics of the ethyl ester of Hyal
(HYAFF7(nw)) exhibited an initial AER about 32% lower and synthesised urea
33% faster than that of cells on collagen films. After a week in culture,
cells on collagen films retained only a minor fraction of their initial rat
es. Cells cultured in non-woven fabrics of HYAFF7(nw) retained about 62 and
44% of their initial AER and USR, respectively, and exhibited an AER appro
ximately equal to and a USR 3.6 times greater than those of cells adherent
to collagen. These results suggest that non-woven fabrics of HYAFF7(nw) are
promising substrata for hepatocyte culture in BALSDs. (C) 2001 Elsevier Sc
ience Ltd. All rights reserved.