The equilibrium constant for the gliotoxin/glutathione pair was found to be
1200+/-100 M-1 at pH 7.0 at 25 degreesC. Under conditions where the reacti
on was quenched rapidly with the addition of acid: gliotoxin-glutathione co
njugate adducts were detected. (C) 2001 Elsevier Science Ltd. All rights re
served.