COMPARISON OF THE INITIAL DISTRIBUTION VOLUME OF GLUCOSE AND SUCROSE IN VOLUME-CHALLENGED DOGS

Objective: Sucrose has been used as an estimate of the extracellular f luid (ECF) volume, This study was designed to examine the hypothesis t hat the initial distribution volume of glucose (IDVG) correlates with the initial distribution volume of sucrose (IDVS) following volume cha llenges, Design: Prospective study for comparison of two methods. Sett ing: Animal laboratory of the University of Hirosaki School of Medicin e. Materials and Methods: Ten mongrel dogs of either sex, weighing 8.5 -14.0 kg, were the subject of the study Dogs were anesthetized with pe ntobarbital and paralyzed with pancuronium, The volume challenge was c onducted with 3% dextran in lactated Ringer's solution 30 ml.kg(-1) ov er 30 min in 10 dogs. Thereafter, the same amount of the fluid was fur ther administered in 8 of these 10 dogs, Before and after each volume challenge, glucose (100 mg.kg(-1)) and sucrose (100 mg.kg(-1)) were in fused simultaneously over 30 s. The two estimates were calculated usin g a one-compartment model from repeated measurements of increased plas ma glucose and sucrose during postinfusion. Results: A linear correlat ion was obtained between the two estimates (r = 0.89, n = 28, p < 0.00 01). All dogs consistently showed that the IDVG individually followed the IDVS during changes in the latter. Conclusions: Results demonstrat ed that the IDVG correlated with the IDVS before and after volume chan ges and support the hypothesis that the IDVG is useful for evaluating the ECF volume status of highly perfused tissues.