Sl. Abidi et Tl. Mounts, REVERSED-PHASE SEPARATIONS OF NITROGENOUS PHOSPHOLIPIDS ON AN OCTADECANOYL POLY(VINYL ALCOHOL) PHASE, Journal of chromatography, 773(1-2), 1997, pp. 93-101
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Molecular species of nitrogenous phospholipids (PLs) phosphatidylcholi
ne (PC), phosphatidylethanolamine (PE), PE-derivatives and sphingomyel
in (SP) were separated on an octadecanoyl poly(vinyl alcohol) (ODPVA)
column by reversed-phase HPLC with UV and evaporative tight scattering
detection (ELSD). Mobile phases employed variable proportions of acet
onitrile, methanol and water. HPLC-UV of the polar Lipids yielded comp
onents with peak intensities somewhat different from those obtained by
HPLC-ELSD despite discernible similarity in the peak profiles observe
d in the two detection systems. Incorporation of ammonium hydroxide in
mobile phases resulted in a decrease in analyte retention. The mobile
phase basicity effect on capacity factors of PE species was significa
ntly greater than that of PC counterparts. The new ODPVA HPLC-ELSD tec
hnique was applied to the analysis of PC and PE molecular species in v
egetable oils. (C) 1997 Elsevier Science B.V.