REVERSED-PHASE SEPARATIONS OF NITROGENOUS PHOSPHOLIPIDS ON AN OCTADECANOYL POLY(VINYL ALCOHOL) PHASE

Molecular species of nitrogenous phospholipids (PLs) phosphatidylcholi ne (PC), phosphatidylethanolamine (PE), PE-derivatives and sphingomyel in (SP) were separated on an octadecanoyl poly(vinyl alcohol) (ODPVA) column by reversed-phase HPLC with UV and evaporative tight scattering detection (ELSD). Mobile phases employed variable proportions of acet onitrile, methanol and water. HPLC-UV of the polar Lipids yielded comp onents with peak intensities somewhat different from those obtained by HPLC-ELSD despite discernible similarity in the peak profiles observe d in the two detection systems. Incorporation of ammonium hydroxide in mobile phases resulted in a decrease in analyte retention. The mobile phase basicity effect on capacity factors of PE species was significa ntly greater than that of PC counterparts. The new ODPVA HPLC-ELSD tec hnique was applied to the analysis of PC and PE molecular species in v egetable oils. (C) 1997 Elsevier Science B.V.