IMPROVED CHIRAL SEPARATION USING ACHIRAL MODIFIERS IN CYCLODEXTRIN MODIFIED CAPILLARY ZONE ELECTROPHORESIS

The influence of achiral modifiers on the chiral separation of propran olol is examined by cyclodextrin modified capillary zone electrophores is. The improved chiral separation of propranolol is by molecules prev iously identified in our group as forming ternary complexes with cyclo dextrin and pyrene. The polarity, chain size and heteroatom compositio n of the functional groups on the comodifiers was systematically varie d in order to study the influence of these variables on the separation of propranolol. The improved chiral separation is accompanied by a de crease in retention time. The decrease in retention time is suggestive of a decrease in the association of beta-cyclodextrin (beta-CD) with propranolol which was verified by calculation of apparent association constants using fluorometric methods.