Structure and conformation of a novel genetically engineered polysaccharide P2

Authors
Colquhoun, IJ Jay, AJ Eagles, J Morris, VJ Edwards, KJ Griffin, AM Gasson, MJ
Citation
Ij. Colquhoun et al., Structure and conformation of a novel genetically engineered polysaccharide P2, CARBOHY RES, 330(3), 2001, pp. 325-333
Citations number
42
Categorie Soggetti
Agricultural Chemistry","Chemistry & Analysis","Organic Chemistry/Polymer Science
Journal title
CARBOHYDRATE RESEARCH
ISSN journal
00086215 → ACNP
Volume
330
Issue
3
Year of publication
2001
Pages
325 - 333
Database
ISI
SICI code
0008-6215(20010215)330:3<325:SACOAN>2.0.ZU;2-N
Abstract
A new exocellular polysaccharide (P2) has been produced by the manipulation of a glycosyl transferase gene (aceP) involved in the biosynthesis of the polysaccharide acetan by the bacterium Acetobacter xylinum strain CKE5. The P2 polysaccharide has been studied by methylation analysis, reductive clea vage, and H-1 and C-13 NMR spectroscopy. The data are consistent with the s tructure predicted when the aceP gene is deactivated: [GRAPHICS] The effect of cooling on proton NMR line width indicates a coil-helix trans ition in P2 at about 70 degreesC. (C) 2001 Elsevier Science Ltd. All rights reserved.