Sequence analysis of the 5 '-terminal part of Glu-D1 locus of landraces and old cultivars of wheat (Triticum aestivum)

We have analyzed 380pb long N-terminal parts of Glu-D1x alleles from severa l lines of landraces and old cultivars of wheat (Triticum aestivum L.) with the aim to verify, whether alleles characterized as identical according to their mobility in SDS-PAGE are encoded always by the same DNA sequences. E uropean landraces and old cultivars were chosen for the study as a potentia l source of higher genetic variability as compared with modern wheat cultiv ars. We have not found any changes in comparison with known data in the seq uences of Dx3 and Dx4 alleles, which in fact were rare in the available set of landraces and old cultivars. Three point mutations were found together in Dx2 and Dx5 alleles of landraces and old cultivars, no one in the standa rd wheat cultivars Chinese Spring and Bezostaya1. Sequencing analysis can b e another suitable technique for study of glutenin alleles complementing SD S-PAGE.