2-3 anti-fungal disease genes are coinserted with hygromycin phosphotransfe
rase in the same vector. Two insecticidal genes and PPT acetyl transferase
genes are placed in another one. The vectors are co-delivered to rice embry
onic cellus tissue at a molar ratio of 1:1 using the particle gun method. 5
5 independent regenerated lines have been obtained through screening for hy
gromycin resistance. Of these, 70% transgenic plants harbor 6-7 foreign gen
es. The genes on the same vectors are always co-delivered to rice plant. No
rthern blot analysis has indicated that the multiple foreign genes give sta
ble expression. In the 6 transgenic plants carrying 6-7 foreign genes, mult
iple foreign genes tend to integrate in 1 or 2 genetic loci. Progeny segreg
ation is consistent with Mendel's 3:1 segregation law. 8 homozygous R-1 tra
nsgenic plants harboring 2-3 anti-fungal and 2 insecticidal genes are selec
ted from large number of transgenic progeny screening for hygromycin and Ba
sta resistance.