Inheritance and expression of multiple disease and insect resistance genesin transgenic rice

2-3 anti-fungal disease genes are coinserted with hygromycin phosphotransfe rase in the same vector. Two insecticidal genes and PPT acetyl transferase genes are placed in another one. The vectors are co-delivered to rice embry onic cellus tissue at a molar ratio of 1:1 using the particle gun method. 5 5 independent regenerated lines have been obtained through screening for hy gromycin resistance. Of these, 70% transgenic plants harbor 6-7 foreign gen es. The genes on the same vectors are always co-delivered to rice plant. No rthern blot analysis has indicated that the multiple foreign genes give sta ble expression. In the 6 transgenic plants carrying 6-7 foreign genes, mult iple foreign genes tend to integrate in 1 or 2 genetic loci. Progeny segreg ation is consistent with Mendel's 3:1 segregation law. 8 homozygous R-1 tra nsgenic plants harboring 2-3 anti-fungal and 2 insecticidal genes are selec ted from large number of transgenic progeny screening for hygromycin and Ba sta resistance.