L. Masana et al., Common genetic variants that relate to disorders of lipid transport in Spanish subjects with premature coronary artery disease, CLIN SCI, 100(2), 2001, pp. 183-190
Fifteen common polymorphic variants at six loci (apolipoproteins Al, B, CII
I and E, hepatic lipase and lipoprotein lipase) involved in plasma lipid tr
ansport have been studied in 210 northern Spanish men, of whom 98 had prove
n coronary artery disease. The other 112 men were clinically free from coro
nary artery disease and acted as controls. The genotypes were investigated
for relationships with plasma lipid and lipoprotein levels, as well as for
the presence of coronary artery disease. As expected, the mean levels of pl
asma triacylglycerols (triglycerides) and lipoprotein (a) and the number of
smokers were significantly higher in the disease group. and high-density l
ipoprotein (HDL)-cholesrerol was significantly lower. Surprisingly, plasma
cholesterol and low-density lipoprotein cholesterol were not different betw
een the two groups. With regard to the common mutations, plasma triacylglyc
erol levels were related to the HindIII variants of lipoprotein lipase (P <
0.05), to the apolipoprotein CIII variant (C3175G in exon 4) and to the ap
olipoprotein Al Xmnl polymorphisms (P < 0.05 and P<0.02 respectively). The
apolipoprotein E variants were related to plasma cholesterol (P(0.05), HDL-
cholesterol (P < 0.02), plasma triacylglycerols (P < 0.05) and the triacylg
lycerol/HDL ratio (P < 0.01). Only the three-codon insertion/deletion varia
nts of the apolipoprotein B signal peptide region discriminated between the
two groups with or without arterial disease (P = 0.02). The possible funct
ional effects of these common mutations are discussed.