Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro

As an extension of our human cDNA project for accumulating sequence informa tion on the coding sequences of unidentified genes, we here present the ent ire sequences of 100 cDNA clones of unidentified genes, named KIAA1673-KIAA 1772, from three sets of size-fractionated cDNA libraries derived from huma n adult whole brain, hippocampus, and fetal whole brain. The average sizes of the inserts and corresponding open reading frames of cDNA clones analyze d here were 4.9 kb and 2.7 kb (corresponding to 895 amino acid residues), r espectively. By computer-assisted analysis of the deduced amino acid sequen ces, 44 predicted gene products were classified into five functional catego ries of proteins relating to cell signaling/communication, nucleic acid man agement, cell structure/motility, protein management, and metabolism. Furth ermore, the expression profiles of the genes were also studied in 10 human tissues, 8 brain regions, spinal cord, fetal brain and fetal liver by rever se-transcription-coupled polymerase chain reaction, the products of which w ere quantified by enzyme-linked immunosorbent assay.