Generation of expressed sequence tags from low-CO2 and high-CO2 adapted cells of Chlamydomonas reinhardtii

To characterize genes whose expression is induced in carbon-stress conditio ns, 12,969 and 13,450 5'-end expressed sequence tags (ESTs) were generated from cells grown in low-CO2 and high-CO2 conditions of the unicellular gree n alga, Chlamydomonas reinhardtii. These ESTs were clustered into 4436 and 3566 non-redundant EST groups, respectively. Comparison of their sequences with those of 3433 non-redundant ESTs previously generated from the cells u nder the standard growth condition indicated that 2665 and 1879 EST groups occurred only in the low-CO2 and high-CO2 populations, respectively. It was also noted that 96.2% and 96.0% of the cDNA species respectively obtained from the low-CO2 and high-CO2 conditions had no similar EST sequence deposi ted in the public databases. The EST species identified only in the low-CO2 treated cells included genes previously reported to be expressed specifica lly in low-CO2 acclimatized cells, suggesting that the ESTs generated in th is study will be a useful source for analysis of genes related to carbon-st ress acclimatization. The sequence information and search results of each c lone will appear at the web site: http://www.kazusa.or.jp/en/plant/chlamy/E ST/.