A chloroplast DegP2 protease performs the primary cleavage of the photodamaged D1 protein in plant photosystem II

Citation
K. Haussuhl et al., A chloroplast DegP2 protease performs the primary cleavage of the photodamaged D1 protein in plant photosystem II, EMBO J, 20(4), 2001, pp. 713-722
Citations number
56
Categorie Soggetti
Molecular Biology & Genetics
Journal title
EMBO JOURNAL
ISSN journal
02614189 → ACNP
Volume
20
Issue
4
Year of publication
2001
Pages
713 - 722
Database
ISI
SICI code
0261-4189(20010215)20:4<713:ACDPPT>2.0.ZU;2-4
Abstract
Although light is the ultimate substrate in photosynthesis, it can also be harmful and lead to oxidative damage of the photosynthetic apparatus, The m ain target for light stress is the central oxygen-evolving photosystem II ( PSII) and its D1 reaction centre protein. Degradation of the damaged D1 pro tein and its rapid replacement by a de novo synthesized copy represent the important repair mechanism of PSII crucial for plant survival under light s tress conditions, Here we report the isolation of a single-copy nuclear gen e from Arabidopsis thaliana, encoding a protease that performs GTP-dependen t primary cleavage of the photodamaged D1 protein and hence catalysing the key step in the repair cycle in plants. This protease, designated DegP2, is a homologue of the prokaryotic Deg/Htr family of serine endopeptidases and is associated with the stromal side of the non-appressed region of the thy lakoid membranes. Increased expression of DegP2 under high salt, desiccatio n and light stress conditions was measured at the protein level.