HsIVU is a two-component ATP-dependent protease, consisting of HsIV peptida
se and HsIU ATPase. CodW and CodX, encoded by the cod operon in Bacillus su
btilis, display 52% identity in their amino acid sequences to HsIV and HsIU
in Escherichia coli, respectively. Here we show that CodW and CodX can fun
ction together as a new type of two-component ATP-dependent protease. Remar
kably, CodW uses its N-terminal serine hydroxyl group as the catalytic nucl
eophile, unlike HsIV and certain P-type subunits of the proteasomes, which
have N-terminal threonine functioning as an active site residue. The ATP-de
pendent proteolytic activity of CodWX is strongly inhibited by serine prote
ase inhibitors, unlike that of HsIVU. Replacement of the N-terminal serine
of CodW by alanine or even threonine completely abolishes the enzyme activi
ty. These results indicate that CodWX in B.subtilis represents the first N-
terminal serine protease among all known proteolytic enzymes.