E. De Alteriis et al., Relation between growth dynamics and diffusional limitations in Saccharomyces cerevisiae cells growing as entrapped in an insolubilised gelatin gel, FEMS MICROB, 195(2), 2001, pp. 245-251
Flow-cytometric analysis was employed to investigate growth dynamics of a y
east cell population immobilised in an insolubilised gelatin gel by means o
f the quantitative determination of the average protein content per cell. T
his analysis was carried out on both the immobilised cell population consid
ered as a whole and the subpopulations colonising the gelatin matrix at dif
ferent depths. The results show that growth of the gelatin-immobilised yeas
t population was affected by the existence of a gradient of nutrient concen
trations through the matrix and are in agreement with the unsteady-state di
ffusion model employed for the description of glucose transfer in the gel.
(C) 2001 Federation of European Microbiological Societies. Published by Els
evier Science B.V. All rights reserved.