Luciferase expression 2 years after DNA injection in glass catfish (Kryptopterus bicirrhus)

Glass catfish (Kryptopterus bicirrhus) have transparent muscles and skin; I ntramuscular injection of DNA encoding luciferase into these fish induced l uciferase expression that was measurable in vivo with a low light video ima ge analyser. Expression could be detected up to at least 2 years after DNA injection. Although luciferase ii; not representative of all types of antig en, this study stresses the need for future studies directed to limit the p eriod of antigen expression after DNA vaccination. (C) 2001 Academic Press.