The response of C-terminal variants of ribonuclease A to pressure and temperature-induced unfolding. A study by fourth derivative UV and Fourier Transform Infrared Spectroscopy

Authors
Torrent, J Connelly, JP Rubens, P Coll, MG Ribo, M Heremans, K Vilanova, M
Citation
J. Torrent et al., The response of C-terminal variants of ribonuclease A to pressure and temperature-induced unfolding. A study by fourth derivative UV and Fourier Transform Infrared Spectroscopy, HIGH PR RES, 19(1-6), 2000, pp. 637-642
Citations number
5
Categorie Soggetti
Physics
Journal title
HIGH PRESSURE RESEARCH
ISSN journal
08957959 → ACNP
Volume
19
Issue
1-6
Year of publication
2000
Part
2
Pages
637 - 642
Database
ISI
SICI code
0895-7959(2000)19:1-6<637:TROCVO>2.0.ZU;2-K
Abstract
The effect of pressure and temperature on ribonuclease A WT and a set of va riants was studied by fourth derivative UV and Fourier Transform Infrared s pectroscopy. The results reveal an unusual similarity between pressure- and temperature-induced unfolding and indicate the importance of the region ex tending from residue 106 to 118 (of 124) in stability and probably in the e arly stages of folding.