Baro-immunodesorption: Development of new affinity supports suitable for semi-continuous processes. Application to the purification of recombinant Escherichia coli beta-galactosidase, and comparison with a conventional purification method

New affinity supports suitable for semi-continous baro-immunodesorption pro cesses were developed. Home made rabbit polyclonal antibodies grafted onto various carriers were used to purify E. coil beta -galactosidase from bacte rial crude extracts. The high pressure desorption method was compared with current elution techniques (i.e., high pH method and competition elution pr ocedure). The properties and the efficiency of the different supports in th e development of a semi-continous elution process are discussed.