Structural prerequisites for the stability of Sso7d from the archaeon Sulfolobus solfataricus versus high pressure and temperature

Authors
Mombelli, E Shehi, E Fusi, P Tortora, P Lange, R Balny, C
Citation
E. Mombelli et al., Structural prerequisites for the stability of Sso7d from the archaeon Sulfolobus solfataricus versus high pressure and temperature, HIGH PR RES, 19(1-6), 2000, pp. 701-706
Citations number
8
Categorie Soggetti
Physics
Journal title
HIGH PRESSURE RESEARCH
ISSN journal
08957959 → ACNP
Volume
19
Issue
1-6
Year of publication
2000
Part
2
Pages
701 - 706
Database
ISI
SICI code
0895-7959(2000)19:1-6<701:SPFTSO>2.0.ZU;2-2
Abstract
Sso7d is a protein purified from the thermoacidophilic archaeon Sulfolobus solfataricus. In this paper we present the effect of pressure and temperatu re on the stability of Sso7d and its mutants. Fourth derivative UV spectros copy and ANS(1-anilinonaphtalene-8-sulfonate) binding were used to monitor the protein unfolding.