Determination of halofuginone and amprolium in chicken muscle and egg by liquid chromatography

A liquid chromatographic (LC) method was developed for simultaneous measure ment of halofuginone (HFN) and amprolium (APL) in chicken muscle and egg. H FN and APL were extracted from chicken muscle and egg with acetonitrile, In chicken egg, they were partially purified by solid-phase extraction (SPE) to separate them from impurities, The LC separation was performed on a 4.5 mm id x 250 mm TSK-gel ODS-80TM column using acetonitrile-McIlvaine buffer, pH 3.4, containing 0.01M sodium lauryl sulfate (42 + 58) as the mobile pha se, Ultraviolet detection of HFN and APL was performed at wavelengths of 24 2 and 255 nm, respectively, Recoveries of HFN and APL from chicken muscle s piked at 0.5 mug/g were 74.8 +/- 17.7 and 94.2 +/- 5.0%, respectively (mean a standard deviation [SD], n= 10), In chicken muscle, the lower limit of d etermination for both APL and HFN was 0.03 mug/g. Recoveries of HFN and APL from chicken egg spiked at 0.5 mug/g by a cleanup procedure using SPE were 54.6 +/- 3.4 and 85.0 +/- 2.4%, respectively (mean +/- SD, n = 5), In chic ken egg, the lower limit of determination for both APL and HFN was 0.04 mug /g.