Evaluation of liquid handling conditions in microplates

Liquid handling in higher density microplates (e.g., 1536-well microplates) for more efficient drug screening necessitates carefully selected and opti mized parameters, The quality of a liquid handling procedure is dependent o n the carryover rate of residual liquids during the pipetting process, the mixing behavior in the wells, foam and bubble formation, and evaporation. W e compared and optimized these parameters in 96-, 384-, and 1536-well micro plates, and herein we critically evaluate the performance of the CyBi(TM)-W ell 96/384/1536 automated micropipetting device, which formed the basis of our evaluation studies.