Identification and quantification of base and nucleoside markers in extracts of Ganoderma lucidum, Ganoderma japonicum and Ganoderma capsules by micellar electrokinetic chromatography

The present paper describes the development of a micellar electrokinetic ch romatographic method for the determination of nucleoside (adenosine, uridin e) and base (uracil) markers in aqueous extracts of Ganoderma medicinal pre parations. The markers were successfully separated within 10 min using an 8 0 mM berate buffer, with 25 mM sodium dodecyl sulfate adjusted to pH 9.0, a n operating voltage of 22 kV, temperature of 20 degreesC and a hydrodynamic injection time of 5 s. Separations were carried out in a fused-silica capi llary with peak detection by direct UV at 254 nm. Following semi-validation of the method, with each analyte showing a good linear relationship over a 0.2 to 20 ppm concentration range (correlation coefficients from 0.9986 to 0.9998), the amounts of the three markers in the various forms of Ganoderm a were easily determined using a relatively simple extraction procedure. (C ) 2001 Elsevier Science B.V. All rights reserved.