Liquid chromatographic enantioseparation of beta-blocking agents with (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isothiocyanate as chiral derivatizing agent

The applicability of (1R,2R)-1,3-diacetoxy-1-(4-nitrophenyl)-2-propyl isoth iocyanate [(R,R)-DANI] as a recently developed chiral derivatizing agent fo r the enantioseparation of a series of beta -blockers is described. The thi ourea diastereomers formed were analyzed by reversed-phase high-performance liquid chromatography, mixtures of water and methanol or acetonitrile bein g used for elution. Conditions of derivatizations (temperature, reagent exc ess and reaction time) were optimized, and the effects of organic modifiers on the retention and separation were investigated; the diastereomers could readily be baseline separated with methanol-containing mobile phases with resolutions between 1.58 and 2.72. (C) 2001 Elsevier Science B.V. All right s reserved.